Construction of a cDNA library involves several steps,the first usually involves:
A)digesting noncoding regions of DNA.
B)cloning complementary double-strand DNA into a plasmid.
C)isolating total RNA from the organism or cell type of interest.
D)reverse transcribing RNA into cDNA.

Question

Quizplus · Accepted Answer

The first step in construction of a cDNA library involves isolating total RNA from the organism or cell type of interest, which is then used as a template to generate cDNA using reverse transcription. Digesting noncoding regions of DNA and cloning complementary double-strand DNA into a plasmid occur later in the library construction process.

Construction of a CDNA Library Involves Several Steps,the First Usually