You have an E.coli plasmid containing a mammalian cDNA and want to isolate the cDNA to use in another experiment.The plasmid map reveals that you can isolate the complete cDNA by digesting the plasmid with EcoRI.How many bands would there be after a successful EcoRI restriction enzyme digest?
A)one
B)two
C)three
D)four

Question

Quizplus · Accepted Answer

There would be two bands after digestion with EcoRI - one corresponding to the plasmid backbone and one corresponding to the isolated cDNA insert.

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