To see if your polymerase chain reaction was successful and it amplified the right sequence of interest,you electrophorese the products from the reaction on an agarose gel.After staining the gel you find there are two bands of different sizes.Which one of the following is a possible explanation for these results?
A)The PCR induced a frame shift mutation into the DNA sequence.
B)RNA polymerase copied the DNA into two copies of RNA.
C)The primers annealed to two different templates.
D)a and b

Question

Quizplus · Accepted Answer

The presence of two bands of different sizes on the gel indicates that the PCR reaction amplified two different DNA templates. This could occur if the primers annealed to two different DNA sequences, leading to the amplification of two different products. A frame shift mutation or RNA polymerase activity would not result in the amplification of two different products.

To See If Your Polymerase Chain Reaction Was Successful and It