Which procedure is most useful in quantifying the active transcription of botulism toxin genes in a can of food that is contaminated?

Question

Quizplus · Accepted Answer

Real-time PCR (also known as qPCR) is the most useful procedure for quantifying the active transcription of botulism toxin genes in a contaminated can of food. Real-time PCR can provide rapid and quantitative measurements of gene expression by monitoring the amplification of specific DNA segments in real-time during the PCR reaction. This technique is highly sensitive and specific, enabling the detection and quantification of low levels of target sequences in complex samples such as food matrices. Southern blotting, end-point PCR, and DNA hybridization are not as sensitive or specific as real-time PCR and may not provide accurate and reliable measurements of gene expression levels in contaminated food samples.

Which Procedure Is Most Useful in Quantifying the Active Transcription