Plasmid DNA having one EcoRI recognition sequence is treated with EcoRI restriction endonuclease.Following gel electrophoresis,how many bands should be visible on the gel?

Question

Quizplus · Accepted Answer

When a plasmid DNA with one EcoRI recognition sequence is treated with EcoRI restriction endonuclease, it will be cut at that specific site, resulting in a linear piece of DNA. Since it was circular and has been cut once, it remains a single linear fragment, thus only one band should be visible on the gel.

Plasmid DNA Having One EcoRI Recognition Sequence Is Treated with EcoRI