In primary mutant screens
A)mutant flies are identifed by morphology and then the genes that are mutated isolated and studied.
B)mutant flies are created by RNAi using random RNA sequences and then the genes affected identified.
C)mutant flies are created by gain-of-function expression of random genes.
D)two mutant flies are mated to determine if their is an affect on morphology.

Question

Quizplus · Accepted Answer

In primary mutant screens, mutant flies are identified based on their morphology, and then the genes that are responsible for the mutated phenotype are isolated and studied. This approach facilitates the identification of genes that are involved in specific biological processes or responses, and can provide insights into the function of previously uncharacterized genes. RNAi is typically used in secondary screens to confirm the role of identified genes in the observed phenotype. Gain-of-function screens are used to identify genes that can enhance or suppress specific biological processes, whereas mating two mutant flies would not necessarily lead to the identification of the affected genes.

In Primary Mutant Screens