How are temperature sensitive mutants used to determine when genes act during development?
A)Embryos can be shifted to the non-permissive temperature to inactivate the mutant gene and the effect of inactivation on subsequent stages observed.
B)Embryos can be shifted to the permissive temperature to inactivate the mutant gene and the effect of inactivation on subsequent stages observed.
C)Adults can be shifted to the non-permissive temperature to inactivate the mutant gene and the effect of inactivation on fertilization can be observed.
D)Embryos can be shifted to the permissive temperature to activate the mutant gene and the effect of activation on subsequent stages observed.

Question

Quizplus · Accepted Answer

Temperature sensitive mutants are able to grow and develop at a permissive temperature, but die or show defects at a non-permissive temperature. By shifting embryos from the permissive temperature to the non-permissive temperature at different stages of development, researchers can determine when the mutant gene is necessary for normal development. This allows the identification of critical periods of gene action during development.

How Are Temperature Sensitive Mutants Used to Determine When Genes