The polymerase chain reaction (PCR)includes all of the following steps except
A)denaturation of primers and the DNA fragment to be amplified.
B)anealing of primers to the complementary sequences on the DNA.
C)primer extension with DNA polymerase.
D)cycle repetition.
E)addition of new Taq polymerase after each cyclE.

Question

Quizplus · Accepted Answer

The addition of new Taq polymerase after each cycle is not a step in the PCR process. The Taq polymerase added at the beginning of the process is sufficient for multiple cycles of denaturation, annealing, and extension. Therefore, E is the correct answer.

The Polymerase Chain Reaction (PCR)includes All of the Following Steps