When electrical current is applied during a gel electrophoresis procedure,the DNA fragments are separated by
A)electrical charge,positive on one side,negatives on the other.
B)the number of poly-A tails associated with each one.
C)their response to the staining chemicals used during the procedure.
D)the size of the fragments.
E)the enzyme binding activity sites.

Question

Quizplus · Accepted Answer

During gel electrophoresis, electrical current is applied to the gel which causes the DNA fragments to move towards the positively charged end. Smaller fragments move faster and travel further than larger fragments, resulting in separation of fragments based on size. Therefore, the best choice is D - the size of the fragments.

When Electrical Current Is Applied During a Gel Electrophoresis Procedure,the