You have isolated a bacterium that you believe to be the causative agent of a new disease in frogs. How would you test the third of Koch's postulates?

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The third postulate of Koch's postulates states that the microbe must be isolated from a diseased organism and grown in pure culture. In this case, the bacterium has already been isolated from a sick frog. To test the third postulate, the bacterium would need to be injected into a healthy frog to see if it causes the same disease. If the frog develops the disease, it would support the third postulate, as the bacterium would have been grown in pure culture outside of the frog and then caused the disease when reintroduced to a healthy host.

You Have Isolated a Bacterium That You Believe to Be