How did Sergei Winogradsky grow lithotrophs?

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Sergei Winogradsky grew lithotrophs using enrichment culture, which involves isolating and growing microorganisms in a specific environment with a particular nutrient or substrate that encourages the growth of the desired organism. This technique allowed him to isolate lithotrophic bacteria and study their metabolism. Aseptic technique is a method to ensure sterile conditions during laboratory work, pure culture refers to a culture consisting of only one type of microbe, endosymbiosis is the process by which one type of organism lives inside another, and chain of infection refers to the sequence of events that lead to the transmission and spread of infectious diseases.

How Did Sergei Winogradsky Grow Lithotrophs