A researcher wants to introduce the human gene encoding tissue plasminogen activator (used to dissolve blood clots)into a mammal so that the protein will be secreted into the milk of the mammary gland.What is required for the researcher's success?

Question

Quizplus · Accepted Answer

The gene should be placed next to the promoter of a gene that is expressed in mammary cells, to allow for expression of the tissue plasminogen activator gene specifically in mammary cells and secretion of the protein into the milk. Placing the gene far from a mammary-specific promoter (A) or replacing a mammary-specific promoter (C) would not lead to optimal expression in mammary cells. Placing the gene next to a promoter expressed in multiple cell types (D) or replacing a promoter expressed in multiple cell types (E) may lead to undesirable off-target effects in other tissues.

A Researcher Wants to Introduce the Human Gene Encoding Tissue