After removal of the RNA primers and replacement with DNA nucleotides,the single-stranded nick adjacent to Okazaki fragments is filled in through a reaction that involves which enzyme?
A)DNA primase
B)SSB protein
C)RNA polymerase
D)DNA ligase
E)DNA helicase

Question

Quizplus · Accepted Answer

The enzyme responsible for filling in the single-stranded nick adjacent to Okazaki fragments is DNA ligase. It catalyzes the formation of phosphodiester bonds between adjacent nucleotides in the DNA backbone. DNA primase synthesizes RNA primers, SSB protein stabilizes single-stranded DNA, RNA polymerase synthesizes RNA from a DNA template, and DNA helicase unwinds double-stranded DNA. None of these enzymes are directly involved in the filling in of the nick.

After Removal of the RNA Primers and Replacement with DNA