Type II restriction enzymes cut
A) double-stranded DNA, forming a 5' phosphoryl group and a 3' hydroxyl group on each strand.
B) single-stranded DNA, forming a 5' phosphoryl group and a 3' hydroxyl group on the strand.
C) double-stranded DNA, forming a 5' phosphoryl group and a 3' hydroxyl group on one strand.
D) double-stranded DNA, forming a 3' phosphoryl group and a 5' hydroxyl group on each strand.
E) single-stranded DNA, forming two hydroxyl groups and loss of a phosphate group.

Question

Quizplus · Accepted Answer

Type II restriction enzymes specifically cut double-stranded DNA, producing a 5' phosphoryl group and a 3' hydroxyl group on each strand, which is a characteristic feature of their action.

Type II Restriction Enzymes Cut