The basis of Sanger DNA sequencing is to
A) identify ESTs and place them in order based on their function to derive the overall sequence.
B) collect all of the mRNAs of a given cell type, make cDNAs from them, align the cDNAs, and derive the overall sequence.
C) break several copies of a genome into 3-base codons, and align them to derive the overall sequence.
D) generate a series of fragments from an overall DNA sequence that differ by the end base of each. Overlap the pieces and read off the overhangs to derive the sequence.
E) tag each of the four DNA bases with a fluorescent marker molecule and align them by the intensity of the fluorescence to derive the overall sequence.

Question

Quizplus · Accepted Answer

Sanger DNA sequencing involves breaking an overall DNA sequence into fragments, each with a different end base, and then overlapping the pieces and reading off the overhangs to derive the sequence. This method was developed by Frederick Sanger and is also known as chain-termination sequencing.

The Basis of Sanger DNA Sequencing Is to