An immunoassay is performed in the following way: Antigen is bound to a solid support.Patient serum is added,and patient antibodies bind to the antigen.The well is washed,and enzyme-labeled anti-immunoglobulin antibodies are added.The well is washed,and enzyme substrate is added.Enzyme activity is determined.Which immunoassay is described?

Question

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This immunoassay is describing the steps of an enzyme-linked immunosorbent assay (ELISA), which uses antigen and antibody interaction to generate a detectable signal. Choice A, capture assays, involves immobilizing antibodies and detecting specific antigens. Choice B, competitive assays, involve competition between labeled and unlabeled antigens for binding to antibodies. Choice D, homogeneous assays, involve detecting the interaction between antigen and antibody in solution without the need for separation steps.

An Immunoassay Is Performed in the Following Way: Antigen Is