A tissue section is fixed to a microscope slide,then incubated with a fluorescently labeled antibody that binds to an antigen expressed by cells in that tissue.The preparation is then washed and inspected with a fluorescent microscope.This technique is called:

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Direct immunofluorescence involves directly labeling the antigen with a fluorescently labeled antibody, which is then visualized with a fluorescent microscope. This is in contrast to indirect immunofluorescence, which involves labeling of a primary antibody with a fluorescently labeled secondary antibody. Fluorescence polarization measures the rotational diffusion of a fluorophore in solution, and autofluorescence refers to the natural fluorescence of some biological molecules.

A Tissue Section Is Fixed to a Microscope Slide,then Incubated