Steps in a Southern blot include which of the following?

Question

Quizplus · Accepted Answer

The first step in a Southern blot is to separate the DNA fragments by gel electrophoresis. The second step is to transfer the pattern from the gel to a membrane or filter.

Answer: A 
 After the transfer, the membrane is incubated with a labeled DNA probe, which binds to complementary sequences in the DNA fragments. The probe is then ligated or covalently linked to the DNA fragments using a DNA ligase.

Answer: C and D 
 These steps are not part of a Southern blot. Denaturation and annealing of primers are part of PCR amplification, which is used for DNA sequencing, cloning, and other applications. Nicking and amplification are part of strand displacement amplification, a technique for amplifying DNA.

Steps in a Southern Blot Include Which of the Following