Question 1

Reverse osmosis is:

Accepted Answer

Reverse osmosis is a process in which water is forced through a semipermeable membrane that acts as a molecular filter, removing impurities and minerals. This results in purified water. Choice A refers to distillation, choice C refers to deionization, and choice D refers to vaporization.

Question 2

What is the molarity (mol/L)of a solution containing 25 g of H₂SO₄ (MW= 98 g)in 150 mL of deionized water?

Accepted Answer

A

Question 3

Regarding centrifugation,what is the formula for calculating relative centrifugal force (RCF)?

Accepted Answer

The formula for calculating relative centrifugal force (RCF) is RCF = 1.118 * 10^-5 * r (in cm) * rpm^2, where r is the radius in centimeters and rpm is the revolutions per minute.

Question 4

You have seen another laboratorian collecting blood from a patient without using any barrier protection.What safety mandate is this employee ignoring?

Accepted Answer

The employee is ignoring the safety mandate of Universal Precautions, which requires the use of barrier protection (such as gloves, gowns, and masks) when coming into contact with bodily fluids or materials that may contain infectious agents. This is a basic safety protocol designed to protect both the patient and healthcare provider from the spread of infection.

Question 5

What is the relative centrifugal force (RCF)for a centrifuge spinning at 1200 rpm with a rotor head radius of 8 inches?

Accepted Answer

The relative centrifugal force (RCF) can be calculated using the formula: RCF = 1.118 x 10^-5 x r x (rpm)^2, where r is the radius in centimeters and rpm is the revolutions per minute. First, convert 8 inches to centimeters: 8 inches = 20.32 cm. Then, RCF = 1.118 x 10^-5 x 20.32 x (1200)^2 ≈ 32.7 g.

Question 6

What is the Clinical and Laboratory Standards Institute (CLSI)recommendation for the most appropriate RCF and time for centrifugation of blood samples to obtain sufficient separation of cellular and liquid components?

Accepted Answer

According to CLSI, the recommended RCF (Relative Centrifugal Force) for centrifugation of blood samples is 1200 g, and the recommended time is 10 min. This centrifugation process is sufficient to separate the cellular and liquid components of the blood sample. Therefore, choice B is the correct answer.

Question 7

The class of fire that involves flammable liquids and gases is class and the recommended extinguisher agent is:

Accepted Answer

Class B fires involve flammable liquids and gases, and the recommended extinguisher agents are dry chemical or carbon dioxide. Water should not be used on this class of fire as it can spread the flames and cause the fire to become more intense. Halon 1211 foam is recommended for Class C fires, which involve electrical equipment.

Question 8

The increased use of electronic health record (EHR)systems has amplified the need for standardized communication between EHR and other systems.The newest universal code system that has standardized terms for all kinds of laboratory observations and measurements and includes a code number,a long formal name,a shorter name,and synonyms is referred to as the:

Accepted Answer

The Logical Observation Identifier Names and Codes (LOINC) system is the newest universal code system that has standardized terms for all kinds of laboratory observations and measurements and includes a code number, a long formal name, a shorter name, and synonyms. The Clinical and Laboratory Standards Institute (CLSI) system is a different standardization system that provides guidelines and recommendations for laboratory best practices. The Occupational Safety and Health Act and National Fire Protection Association systems are not relevant to this topic.

Question 9

The one technical aspect of a clinical laboratory that has received considerable attention in the need for ergonomic design to decrease musculoskeletal stress would be:

Accepted Answer

Pipetting involves repetitive motions and sustained postures, which can lead to musculoskeletal disorders. Ergonomic pipettes and workstations, as well as proper training and scheduling of breaks, can help decrease this stress. While chair design, lighting, and flooring are important considerations for overall laboratory safety and efficiency, they do not directly address musculoskeletal stress.

Question 10

A highly purified chemical that is directly weighed or measured to produce a solution whose concentration is exactly known is referred to as a:

Accepted Answer

A primary reference material is a highly purified chemical that is directly weighed or measured to produce a solution whose concentration is exactly known. Calibrators are also used in analytical chemistry, but they are not necessarily directly weighed, and their concentration may not be exactly known. Secondary standards are materials that are calibrated against primary reference materials. Working solutions are solutions prepared from primary or secondary standards to be used for analysis, but they are not directly weighed or measured like primary reference materials.

Question 11

Chemicals with the designation _____ are suitable for use in most clinical chemistry laboratory analyses.

Accepted Answer

Analytical reagent grade chemicals have the highest purity and are suitable for laboratory analytical purposes, including clinical chemistry laboratory analyses. Technical grade chemicals may contain impurities that can affect the accuracy and reliability of laboratory analyses. USP and NF grades are used exclusively for pharmaceutical manufacturing and not necessary for laboratory analyses.

Question 12

The maximum amount of working volume of a flammable solvent allowed outside a storage cabinet is 1 gallon per room.

Accepted Answer

The maximum amount of flammable solvent allowed outside a storage cabinet varies depending on various factors such as the type of solvent, the type of container, and the fire protection system in place. However, there is no universally established limit of 1 gallon per room. It is important to follow the safety guidelines set by the relevant regulatory bodies and ensure proper handling, storage, and use of flammable solvents.

Question 13

Universal Precautions:

Accepted Answer

Universal Precautions consist of guidelines that necessitate the wearing of barrier protection such as gloves, gowns, and face masks when handling potentially infectious material to prevent the transmission of bloodborne pathogens. Options A and B describe shipping standards and specimen handling procedures, respectively, but do not encompass the full scope of Universal Precautions. Option C is incorrect as hand washing is necessary before and after any patient contact, not just when entering a clinical laboratory.

Question 14

Which one of the following statements concerning type I water is not correct?

Accepted Answer

Type I water is obtained from a combination of multiple purification methods, including ion exchange purification, reverse osmosis, and additional purification steps such as ultraviolet or electrodeionization. Type I water is required for sensitive analytical applications, but it may still contain trace amounts of impurities.

Question 15

What is the normality of a solution containing 20 g of H₂SO₄ (MW = 98)in 500 mL of buffer?

Accepted Answer

First, we need to calculate the number of moles of H₂SO₄ in the solution: n = m/MW = 20 g / 98 g/mol = 0.204 mole Next, we need to determine the volume of the solution in liters: 500 mL = 0.5 L Finally, we can calculate the normality (N) using the formula: N = (equivalents of solute) / (volume of solution in liters) Since H₂SO₄ can donate one hydrogen ion (H+) in solution, it has one equivalent per mole. Therefore, the number of equivalents in the solution is equal to the number of moles of H₂SO₄. N = 0.204 eq / 0.5 L = 0.408 N ≈ 0.82 N. Therefore, the best choice is C, with a normality of 0.82 N.

Question 16

To make 20 mL of a 0.1 g/L solution from a 10 mg/mL stock solution,you should add _____ mL of the stock solution to _____ mL of solvent.

Accepted Answer

To make a 0.1 g/L solution, you need 0.002 g (0.1 g/L * 0.02 L) of solute. The stock solution is 10 mg/mL, which is 0.01 g/mL. Therefore, you need 0.2 mL of the stock solution (0.002 g / 0.01 g/mL) and 19.8 mL of solvent to make 20 mL of the desired solution.

Question 17

The type of pipette that is a piece of glass tubing drawn out to a tip and graduated uniformly along its length and is used for reagent measurement is referred to as a:

Accepted Answer

A measuring pipette is designed with graduations along its length for measuring varying volumes of liquid, unlike volumetric pipettes which are calibrated for a single volume.

Question 18

The National Institute for Occupational Safety and Health of the CDC distributes a document that specifies how U.S.clinical laboratories should handle infectious agents.This document is referred to as:

Accepted Answer

The answer of The National Institute for Occupational Safety and...

Question 19

Fifty milligrams (mg)per 1000 mL is equal to:

Accepted Answer

The answer of Fifty milligrams (mg)per 1000 mL is equal...

Question 20

How many grams of NaCl (MW = 58.5 g)are required to prepare 250 mL of a 6 mol/L solution of NaCl?

Accepted Answer

To prepare a 6 mol/L solution, you need 6 moles of NaCl per liter. Since the question asks for a 250 mL (0.25 L) solution, you multiply 6 moles/L by 0.25 L to get 1.5 moles of NaCl. Then, multiply the number of moles (1.5) by the molar mass of NaCl (58.5 g/mol) to find the mass required: 1.5 moles * 58.5 g/mol = 87.75 g.

Quiz 8: Principles of Basic Techniques and Laboratory Safety

Reverse osmosis is:

What is the molarity (mol/L) of a solution containing 25 g of H₂SO₄ (MW= 98 g) in 150 mL of deionized water?

Regarding centrifugation,what is the formula for calculating relative centrifugal force (RCF) ?

You have seen another laboratorian collecting blood from a patient without using any barrier protection.What safety mandate is this employee ignoring?

What is the relative centrifugal force (RCF) for a centrifuge spinning at 1200 rpm with a rotor head radius of 8 inches?

What is the Clinical and Laboratory Standards Institute (CLSI) recommendation for the most appropriate RCF and time for centrifugation of blood samples to obtain sufficient separation of cellular and liquid components?

The class of fire that involves flammable liquids and gases is class and the recommended extinguisher agent is:

The one technical aspect of a clinical laboratory that has received considerable attention in the need for ergonomic design to decrease musculoskeletal stress would be:

A highly purified chemical that is directly weighed or measured to produce a solution whose concentration is exactly known is referred to as a:

Chemicals with the designation _____ are suitable for use in most clinical chemistry laboratory analyses.

The maximum amount of working volume of a flammable solvent allowed outside a storage cabinet is 1 gallon per room.

Universal Precautions:

Which one of the following statements concerning type I water is not correct?

What is the normality of a solution containing 20 g of H₂SO₄ (MW = 98) in 500 mL of buffer?

To make 20 mL of a 0.1 g/L solution from a 10 mg/mL stock solution,you should add _ mL of the stock solution to _ mL of solvent.

The type of pipette that is a piece of glass tubing drawn out to a tip and graduated uniformly along its length and is used for reagent measurement is referred to as a:

The National Institute for Occupational Safety and Health of the CDC distributes a document that specifies how U.S.clinical laboratories should handle infectious agents.This document is referred to as:

Fifty milligrams (mg) per 1000 mL is equal to:

How many grams of NaCl (MW = 58.5 g) are required to prepare 250 mL of a 6 mol/L solution of NaCl?

Quiz 8: Principles of Basic Techniques and Laboratory Safety

Reverse osmosis is:

What is the molarity (mol/L) of a solution containing 25 g of H2SO4 (MW= 98 g) in 150 mL of deionized water?

Regarding centrifugation,what is the formula for calculating relative centrifugal force (RCF) ?

You have seen another laboratorian collecting blood from a patient without using any barrier protection.What safety mandate is this employee ignoring?

What is the relative centrifugal force (RCF) for a centrifuge spinning at 1200 rpm with a rotor head radius of 8 inches?

What is the Clinical and Laboratory Standards Institute (CLSI) recommendation for the most appropriate RCF and time for centrifugation of blood samples to obtain sufficient separation of cellular and liquid components?

The class of fire that involves flammable liquids and gases is class and the recommended extinguisher agent is:

The one technical aspect of a clinical laboratory that has received considerable attention in the need for ergonomic design to decrease musculoskeletal stress would be:

A highly purified chemical that is directly weighed or measured to produce a solution whose concentration is exactly known is referred to as a:

Chemicals with the designation _____ are suitable for use in most clinical chemistry laboratory analyses.

The maximum amount of working volume of a flammable solvent allowed outside a storage cabinet is 1 gallon per room.

Universal Precautions:

Which one of the following statements concerning type I water is not correct?

What is the normality of a solution containing 20 g of H2SO4 (MW = 98) in 500 mL of buffer?

To make 20 mL of a 0.1 g/L solution from a 10 mg/mL stock solution,you should add _____ mL of the stock solution to _____ mL of solvent.

The type of pipette that is a piece of glass tubing drawn out to a tip and graduated uniformly along its length and is used for reagent measurement is referred to as a:

The National Institute for Occupational Safety and Health of the CDC distributes a document that specifies how U.S.clinical laboratories should handle infectious agents.This document is referred to as:

Fifty milligrams (mg) per 1000 mL is equal to:

How many grams of NaCl (MW = 58.5 g) are required to prepare 250 mL of a 6 mol/L solution of NaCl?

What is the molarity (mol/L) of a solution containing 25 g of H₂SO₄ (MW= 98 g) in 150 mL of deionized water?

What is the normality of a solution containing 20 g of H₂SO₄ (MW = 98) in 500 mL of buffer?

To make 20 mL of a 0.1 g/L solution from a 10 mg/mL stock solution,you should add _ mL of the stock solution to _ mL of solvent.