A manual stat protein analysis is performed on a patient sample.The patient's unknown sample had an absorbance reading of 0.6.The protein calibrator with an absorbance reading closest to the unknown's absorbance had a concentration of 6 g/dL and an absorbance reading of 0.5.Applying Beer law,calculate the unknown protein concentration in the patient sample.

Question

Quizplus · Accepted Answer

According to Beer's law, the concentration of a solution is directly proportional to its absorbance. Mathematically, this can be expressed as A = εcl, where A is the absorbance, ε is the molar absorptivity, c is the concentration and l is the path length of the cuvette.

We can use Beer's law to calculate the concentration of the unknown protein solution as follows:

- A1/A2 = C1/C2, where A1 and A2 are the absorbances of the unknown and the calibrator, respectively, and C1 and C2 are their concentrations. 
- Solving for C1, we get C1 = (A1/A2) x C2 = (0.6/0.5) x 6 g/dL = 7.2 g/dL

Therefore, the best choice is B, which is 7.2 g/dL.

A Manual Stat Protein Analysis Is Performed on a Patient