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When Selecting a Photometric Technique That Is More Sensitive to a Low

Question 17

Multiple Choice

When selecting a photometric technique that is more sensitive to a low concentration of analyte,a laboratory director compares absorbance measuring devices against those that measure fluorescence intensity.Which technique is considered more sensitive in its measurements and why?


A) Absorbance measurements are more sensitive because of the ability of the monochromator to isolate very specific parts of the spectrum.
B) Fluorescence intensity measurements are more sensitive because the fluorophores used are very specific to the analytes they bind to and will bind to small analytes easily.
C) Fluorescence intensity measurements are more sensitive because of the use of intense light sources,signal filtering,and sensitive light emission photometers.
D) Absorbance measurements are more sensitive because the flame can break down complex compounds into elements that are specifically measured.

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