There Are Several Interferences That Can Affect or Limit Fluorescence

There are several interferences that can affect or limit fluorescence measurements.For example,the inner filter effect:

A) occurs when the solution absorbs greater than 2% of the exciting light,producing a nonlinear response between concentration and fluorescence emission.
B) involves fluorophores that have overlapping excitation and emission spectra and that are susceptible to loss of detection because of background light scatter.
C) involves solvents such as ethanol that cause appreciable fluorescence due to the interaction of the fluorophore with the solvent.
D) is caused by light scattering of proteins and other macromolecules in the sample matrix,which might cause unwanted background fluorescence.