In a fluorometric measurement,the difference between the maximum wavelength of the excitation light and the maximum wavelength of the emitted fluorescence light is a constant referred to as:

Question

Quizplus · Accepted Answer

The constant difference between the maximum wavelength of the excitation light and the maximum wavelength of the emitted fluorescence light is called the Stokes shift. This shift occurs due to the relaxation of energy during the excitation-emission process, and is an important characteristic used in fluorometric measurements. The other options (A, B, and C) do not accurately describe this phenomenon.

In a Fluorometric Measurement,the Difference Between the Maximum Wavelength of the Excitation