In the initial step of the diazo reaction for direct bilirubin determination:
A) the absorption of light by bilirubin near 460 nm is measured.
B) bilirubin fractions are oxidized to biliverdin,which is further oxidized to purple.
C) serum is added to an aqueous solution of caffeine,sodium acetate,and sodium benzoate.
D) large quantities of ascorbic acid worsen the detection limit.
Correct Answer:
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