Which of the following procedures can be used to avoid contamination with PCR reaction product (amplicon)when performing a PCR procedure?

Question

Quizplus · Accepted Answer

All three choices can be used to avoid contamination with PCR reaction product (amplicon). Positive displacement pipettes prevent contamination by ensuring that the sample is aspirated and dispensed using a disposable tip, preventing any carryover of previous samples. Closed-tube methods prevent contamination by preventing any aerosol contamination during the opening or closing of tubes. Physical separation of preamplification and postamplification rooms prevents contamination by isolating the pre- and post-amplification areas to prevent cross-contamination. Using all three methods together provides the most comprehensive protection against contamination.

Which of the Following Procedures Can Be Used to Avoid