Real-time PCR, also known as quantitative PCR (qPCR), allows for the monitoring of the amplification of nucleic acid fragments in real-time as they are synthesized. This is achieved by using fluorescent dyes or probes that emit a signal when bound to the amplified product, allowing for the quantification of the target DNA or RNA. Options A, C, and D are not accurate descriptions of real-time PCR.

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In Real-Time PCR

Question 16

Multiple Choice

In real-time PCR:

A) an isolated mRNA and Thermus thermophilus is used.
B) one can observe amplified nucleic acid fragments as they are synthesized.
C) RNA:DNA hybrids are bound to a solid phase.
D) RNA targets are used in an isothermal reaction.

Correct Answer: