What is the purpose of the labeled oligonucleotide probe used in hybridization assays?

Question

Quizplus · Accepted Answer

The purpose of a labeled oligonucleotide probe in hybridization assays is to detect the presence of a specific nucleotide sequence. The probe is designed to be complementary to the target sequence and will bind to it if it is present in the sample being tested. The label on the probe allows for its detection and visualization, indicating the presence or absence of the target sequence. Option A, stabilizing the assay, is not the primary purpose of the labeled oligonucleotide probe, as this is primarily achieved through proper assay design and conditions. Option B, cutting the DNA into smaller-size fragments, is not the purpose of the probe, as this is usually done using restriction enzymes or fragmentation methods prior to the hybridization step. Option C, reducing background, may be achieved through the use of blocking agents or wash steps, but this is not the primary purpose of the labeled oligonucleotide probe.

What Is the Purpose of the Labeled Oligonucleotide Probe Used