Which of the following methods could be used to produce a F_ab fragment from an IgG? A)treatment with reducing agent, isolation of the light chain, removal of reducing agent B)treatment with reducing agent, isolation of the heavy chain, removal of reducing agent C)treatment with papain, separation of the F_ab fragment from the rest of the heavy chain D)treatment with papain, separation of the F_ab fragment from the rest of the light chain E)none of the above

Question

Quizplus · Accepted Answer

Papain specifically cleaves the hinge region of IgG, producing two F(ab')2 fragments and a single Fc fragment. Treatment with papain would result in production of the F_ab fragment, which could then be separated from the rest of the heavy chain.

Which of the Following Methods Could Be Used to Produce