Which of these techniques uses antibodies to detect very small amounts of specific proteins following separation by SDS-PAGE.
A)immunoblotting
B)silverstaining
C)Coomassie Brilliant Blue staining
D)ELISA
E)RIA

Question

Quizplus · Accepted Answer

Immunoblotting, also known as western blotting, uses specific antibodies to detect proteins separated by SDS-PAGE. This technique is very sensitive and can detect very small amounts of specific proteins. Silver staining and Coomassie Brilliant Blue staining are commonly used to visualize proteins in gel assays but do not involve antibodies. ELISA and RIA are also immunodetection techniques but are not specific to SDS-PAGE separation.

Which of These Techniques Uses Antibodies to Detect Very Small