Disulfide bonds can be cleaved using
A)iodoacetate.
B)dansyl chloride.
C)2-mercaptoethanol ($\beta$-ME).
D)trypsin.
E)phenylisothiocyanate.

Question

Quizplus · Accepted Answer

2-mercaptoethanol (β-ME) is a reducing agent commonly used to cleave disulfide bonds by breaking the S-S bond and forming free thiol groups. This reaction is reversible, and if the disulfide bonds are desired again, they can be generated by oxidation with an appropriate oxidizing agent. The other options listed are not commonly used for cleaving disulfide bonds.

Disulfide Bonds Can Be Cleaved Using A)iodoacetate β\betaβ -ME) D)trypsin

Disulfide Bonds Can Be Cleaved Using
A)iodoacetate $\beta$ -ME)
D)trypsin