A researcher is attempting to replicate DNA in vitro.He adds all of the components to the tube except Mg²⁺.What kind of result can he expect to observe? A) The DNA will replicate at a normal rate B) The DNA will replicate but at a much slower rate C) The DNA will not replicate because DNA polymerase will not be active D) The DNA will not replicate because DNA polymerase was not added E) The DNA will not replicate because the Mg²⁺ is what catalyzes the DNA synthesis

Question

Quizplus · Accepted Answer

Mg²⁺ refers to magnesium ions and dNTPs (deoxyribonucleotide triphosphates) which are essential co-factors for DNA polymerase activity. Without these co-factors, DNA polymerase cannot function and DNA replication will not occur. Therefore, the researcher can expect that the DNA will not replicate because DNA polymerase will not be active.

A Researcher Is Attempting to Replicate DNA in Vitro