A researcher is attempting to replicate DNA in vitro.All necessary components are added,however,the DNA that is used does not have a free 3' hydroxyl.What kind of result is expected?
A) The DNA will replicate at a normal rate
B) The DNA will replicate but at a much slower rate
C) The DNA will not replicate because DNA polymerase will not be active
D) The DNA will not replicate because DNA polymerase can not add dNTPs to the existing DNA
E) The DNA will not replicate because the free 3'hydroxyl is what catalyzes the DNA synthesis

Question

Quizplus · Accepted Answer

DNA polymerase requires a free 3' hydroxyl group to add nucleotides to a growing DNA strand. Without it, the enzyme cannot attach new nucleotides, halting replication.

A Researcher Is Attempting to Replicate DNA in Vitro