Two Bacterial Strains Were Obtained with the Following Genotypes After an Uninterrupted Conjugation,you Want to Select F^- Recombinants That

Two bacterial strains were obtained with the following genotypes: $\begin{array}{|l|l|} \hline\quad\quad\quad\quad\quad& \text { Hfr: alaleu }{ }^{+} a\_{-1} \mathrm{St}^{2} \mathrm{r}^{5} \quad\quad\quad\quad\quad\quad\quad\\ \hline&F^{-}: \quad a l a^{+} l e u r a-l^{R} S t r^{R} \\\hline\end{array}$

After an uninterrupted conjugation,you want to select F^- recombinants that are ala⁺leu⁺.Which of the following media would you use for this selection?

A) complete medium containing streptomycin
B) minimal medium containing streptomycin
C) minimal medium containing leucine and streptomycin
D) minimal medium containing sodium azide and leucine
E) minimal medium containing alanine,leucine,and streptomycin