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An EColi F- Strain Has the Following Genotype: S-U-R-I-E-C- The Positions of Hfr 2,the Gene C,and the Polarity of Hfr

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An E.coli F- strain has the following genotype: S-U-R-I-E-C-.Three different Hfr strains,all carrying S+U+R+I+E+ and C+ markers and all having lambda phages integrated at the same specific site in their chromosomes are mated with the F strain in separate matings.The interrupted-mating results are given below.The numbers indicate time (in minutes)when different donor markers appeared in F- cells after conjugation began.Assume that the E.coli map consists of 100 minutes,and gene C is mapped at 80 minutes.
 Marker’S  Hfr 1  Hfr 2  Hfr 3R1020I405U255E60C45S5520\begin{array}{llll}\text { Marker'S } & \text { Hfr 1 } &\text { Hfr 2 } & \text { Hfr } 3\\\hline R & 10 & 20 & - \\I & 40 & - & 5 \\U & 25 & 5 & - \\E & - & 60 & - \\C & - & 45 & - \\S & 55 & - & 20\end{array}

The positions of Hfr 2,the gene C,and the polarity of Hfr 2 are given in the accompanying map.  An E.coli F<sup>-</sup> strain has the following genotype: S<sup>-</sup>U<sup>-</sup>R<sup>-</sup>I<sup>-</sup>E<sup>-</sup>C<sup>-</sup>.Three different Hfr strains,all carrying S<sup>+</sup>U<sup>+</sup>R<sup>+</sup>I<sup>+</sup>E<sup>+</sup> and C<sup>+</sup> markers and all having lambda phages integrated at the same specific site in their chromosomes are mated with the F strain in separate matings.The interrupted-mating results are given below.The numbers indicate time (in minutes)when different donor markers appeared in F<sup>-</sup> cells after conjugation began.Assume that the E.coli map consists of 100 minutes,and gene C is mapped at 80 minutes.   \begin{array}{llll} \text { Marker'S } & \text { Hfr 1 } &\text { Hfr 2 } & \text { Hfr } 3\\ \hline R & 10 & 20 & - \\ I & 40 & - & 5 \\ U & 25 & 5 & - \\ E & - & 60 & - \\ C & - & 45 & - \\ S & 55 & - & 20 \end{array}    The positions of Hfr 2,the gene C,and the polarity of Hfr 2 are given in the accompanying map.    a)Give the gene order,starting from C and going clockwise. b)What is the location of the origin of Hfr 1? Hfr 3? c)What is the location of the I marker? The S marker? d)What is the location of the lambda phage in these strains?
a)Give the gene order,starting from C and going clockwise.
b)What is the location of the origin of Hfr 1? Hfr 3?
c)What is the location of the I marker? The S marker?
d)What is the location of the lambda phage in these strains?

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a)CRUISE (if counterclockwise,...

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