An EColi F- Strain Has the Following Genotype: S-U-R-I-E-C- The Positions of Hfr 2,the Gene C,and the Polarity of Hfr
An E.coli F- strain has the following genotype: S-U-R-I-E-C-.Three different Hfr strains,all carrying S+U+R+I+E+ and C+ markers and all having lambda phages integrated at the same specific site in their chromosomes are mated with the F strain in separate matings.The interrupted-mating results are given below.The numbers indicate time (in minutes)when different donor markers appeared in F- cells after conjugation began.Assume that the E.coli map consists of 100 minutes,and gene C is mapped at 80 minutes.
The positions of Hfr 2,the gene C,and the polarity of Hfr 2 are given in the accompanying map.
a)Give the gene order,starting from C and going clockwise.
b)What is the location of the origin of Hfr 1? Hfr 3?
c)What is the location of the I marker? The S marker?
d)What is the location of the lambda phage in these strains?
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