In eukaryotic DNA replication,re-association of histones with newly formed DNA is accomplished by:
A)spontaneous association of histones with DNA.
B)chromatin assembly factor (CAF-1)attaching to the primase enzyme.
C)histones only associating with new DNA after the chromosome is completely replicated.
D)histones not releasing DNA during replication.
E)chromatin assembly factor 1 (CAF-1)and histones binding to the sliding clamp structure.

Question

Quizplus · Accepted Answer

The re-association of histones with newly formed DNA is accomplished by chromatin assembly factor 1 (CAF-1) and histones binding to the sliding clamp structure. This allows for efficient and accurate assembly of chromatin on the newly synthesized DNA strands. Choice A is incorrect because histones do not spontaneously associate with DNA, but are actively guided by protein factors. Choice B is incorrect because CAF-1 does not attach to the primase enzyme, but rather interacts with the sliding clamp. Choice C is incorrect because histones can associate with new DNA as it is being synthesized, not just after the entire chromosome has finished replicating. Choice D is incorrect because histones must release DNA during replication in order for the DNA polymerase to access the DNA template.

In Eukaryotic DNA Replication,re-Association of Histones with Newly Formed DNA