The experimental value of a "pulse-chase" cellular labeling experiment using radioactive uracil is that:
A)all RNAs within a cell are labeled and easily detected.
B)the radioactivity allows for easy purification of cellular nucleic acids.
C)all RNAs created during the pulse are labeled and detectable,revealing their stability and localization.
D)the spliceosome function is easily seen with radiolabeled RNAs in the cellular cytosol.
E)cells adapt to the pulse of radioactivity,revealing nucleic acid repair mechanisms.

Question

Quizplus · Accepted Answer

In a pulse-chase experiment, cells are initially exposed to a radioactive nucleotide during the "pulse" period, allowing for labeling of newly synthesized RNAs. After the pulse period, the radioactive nucleotide is removed and replaced with a non-radioactive one during the "chase" period, allowing for detection and analysis of the labeled RNAs over time. This experimental approach is particularly useful for examining RNA stability and localization, as it allows for the detection of newly synthesized RNAs (during the pulse) and their fate over time (during the chase). Therefore, choice C is the correct answer, as it accurately reflects one of the main advantages of pulse-chase experiments with radioactive uracil.

The Experimental Value of a "Pulse-Chase" Cellular Labeling Experiment Using