The intermediate temperature cycle (72°C)in the polymerase chain reaction enables:
A)hybridization between the template DNA and the PCR primers.
B)denaturation of the template DNA to enable primer access to matching sequence.
C)activation of the primers to being the amplification procedure.
D)activity of the thermostable DNA polymerase enzyme.
E)a reduction of contamination in the amplification reaction.

Question

Quizplus · Accepted Answer

The intermediate temperature cycle (72°C) in PCR is optimal for the activity of the thermostable DNA polymerase enzyme, which extends the primers to synthesize the new DNA strands.

The Intermediate Temperature Cycle (72°C)in the Polymerase Chain Reaction Enables