Which of the following about the Sanger sequencing method is/are TRUE?
A)Sanger sequencing uses dideoxynucleotide triphosphates to terminate DNA polymerization.
B)Dideoxynucleotides lack 2′ and 3′ OH groups in the ribose sugar.
C)All four deoxynucleotide triphosphates are used in a Sanger sequencing reaction.
D)Fluorescent dyes can be used to automate Sanger sequencing.
E)All of the answer options are correct.

Question

Quizplus · Accepted Answer

All of the answer options are correct. Sanger sequencing uses dideoxynucleotide triphosphates (ddNTPs) to terminate DNA polymerization. These ddNTPs lack a 3′ hydroxyl group, which prevents further extension of the DNA strand. All four of the standard deoxynucleoside triphosphates (dNTPs) are used in Sanger sequencing reactions, along with the ddNTPs. Fluorescent dyes can be used to label and automate the sequencing reactions, leading to faster and more efficient sequencing.

Which of the Following About the Sanger Sequencing Method Is/are