Detection of a transgenic plant can be accomplished in a number of different ways,but often the initially transformed cells (in tissue culture)are enriched for the presence of a transgene by:
A)screening groups of cells using the polymerase chain reaction.
B)examining if the phenotype of the cells has been altered.
C)placing the potentially transformed cells in the presence of a drug that inhibits the division of non-transgenic cells.
D)microscopically examining the cells and isolating those that have "extra" DNA.
E)None of the above.Transgenesis cannot be selected for in tissue culture.

Question

Quizplus · Accepted Answer

By placing the potentially transformed cells in the presence of a drug that inhibits the division of non-transgenic cells, only the transformed cells would survive and divide, thereby enriching for the presence of the transgene. This method is often used in the selection of transformed plant cells in tissue culture. Option A is also possible, but it involves screening groups of cells using the polymerase chain reaction, which can be time-consuming and expensive. Option B is not reliable as not all transgenic plants display a visible phenotype. Option D is not practical as it is difficult to isolate cells with "extra" DNA in a mixture of many cells in tissue culture. Option E is not accurate as transgenesis can be selected for in tissue culture.

Detection of a Transgenic Plant Can Be Accomplished in a Number