In Southern and Northern blotting,the probe being used to analyze DNA or RNA identifies the target sequence via:
A)attaching to the gene target via antibody-like protein-to-protein interactions.
B)a DNA ligase-mediated attachment to the target DNA or RNA fragments.
C)annealing between complementary sequences on the probe and the gene target.
D)an enzymatic step that includes crosslinking between similar nucleic acid sequences.
E)amplification during the polymerase chain reaction.

Question

Quizplus · Accepted Answer

In Southern and Northern blotting, the probe is a single-stranded nucleic acid molecule labeled with a radioactive or fluorescent tag. The probe is designed to be complementary to the target DNA or RNA sequence of interest. This complementary annealing between the probe and the gene target allows for the identification and detection of the target sequence. The other answer choices are not accurate descriptions of the probe-target interaction in Southern or Northern blotting.

In Southern and Northern Blotting,the Probe Being Used to Analyze