You purified genomic DNA from tissue isolated from a population of roundworms (C.elegans).You wish to analyze this DNA,so you digest a portion of this DNA with the restriction enzyme EcoRI,and another portion of the DNA is digested with SalI.You make a gel made of agarose,with wells for loading your DNA.You place undigested DNA in well "A," the EcoRI digested DNA in well "B," and the SalI digested DNA in well "C." An electric field is generated by placing an anode (+ electrode)at the base of the gel,and a cathode (- electrode)at the top of the gel near the wells.The DNA migrates towards the anode.
a)Describe the movement of the DNA along the gel.How is an electric field able to generate movement of the DNA fragments?
b)How can nucleic acids be visualized in an agarose gel?
c)How would you expect lane A,B,and C to appear?
d)Can you analyze the size of fragments that contain a particular gene?

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The Answer of You purified genomic DNA from tissue isolated from a population of roundworms (C.elegans).You wish to analyze this DNA,so you digest a portion of this DNA with the restriction enzyme EcoRI,and another portion of the DNA is digested with SalI.You make a gel made of agarose,with wells for loading your DNA.You place undigested DNA in well "A," the EcoRI digested DNA in well "B," and the SalI digested DNA in well "C." An electric field is generated by placing an anode (+ electrode)at the base of the gel,and a cathode (- electrode)at the top of the gel near the wells.The DNA migrates towards the anode.
a)Describe the movement of the DNA along the gel.How is an electric field able to generate movement of the DNA fragments?
b)How can nucleic acids be visualized in an agarose gel?
c)How would you expect lane A,B,and C to appear?
d)Can you analyze the size of fragments that contain a particular gene?

You Purified Genomic DNA from Tissue Isolated from a Population