The restriction enzyme ClaI recognizes the sequence AT $\times$ CGAT.It cleaves the phosphodiester backbone between the T and the C bases at the arrow.The restriction enzyme TaqI recognizes the sequence T ↓ CGA.It cleaves the phosphodiester backbone between the T and the C bases at the arrow.
a)Indicate the ends of the double-stranded DNA molecule left after cleavage by ClaI.Show the sequence,polarity,and overhang,if any.
b)On average,how many sites would TaqI recognize in a random sequence of 1200 base pairs?
c)If a TaqI-digested DNA end is ligated to a ClaI-digested DNA end,will ClaI cleave the newly ligated DNA? Will TaqI cleave the newly ligated DNA?

Question

The restriction enzyme ClaI recognizes the sequence AT $\times$ CGAT.It cleaves the phosphodiester backbone between the T and the C bases at the arrow.The  restriction enzyme TaqI recognizes the sequence T ↓ CGA.It cleaves the phosphodiester backbone between the T and the C bases at the arrow.
a)Indicate the ends of the double-stranded DNA molecule left after cleavage by ClaI.Show the sequence,polarity,and overhang,if any.
b)On average,how many sites would TaqI recognize in a random sequence of 1200 base pairs?
c)If a TaqI-digested DNA end is ligated to a ClaI-digested DNA end,will ClaI cleave the newly ligated DNA? Will TaqI cleave the newly ligated DNA?

Quizplus · Accepted Answer

The Answer of The restriction enzyme ClaI recognizes the sequence AT $\times$ CGAT.It cleaves the phosphodiester backbone between the T and the C bases at the arrow.The  restriction enzyme TaqI recognizes the sequence T ↓ CGA.It cleaves the phosphodiester backbone between the T and the C bases at the arrow.
a)Indicate the ends of the double-stranded DNA molecule left after cleavage by ClaI.Show the sequence,polarity,and overhang,if any.
b)On average,how many sites would TaqI recognize in a random sequence of 1200 base pairs?
c)If a TaqI-digested DNA end is ligated to a ClaI-digested DNA end,will ClaI cleave the newly ligated DNA? Will TaqI cleave the newly ligated DNA?

The Restriction Enzyme ClaI Recognizes the Sequence AT ×\times× CGATIt Cleaves the Phosphodiester Backbone Between the T and the Cleaves

The Restriction Enzyme ClaI Recognizes the Sequence AT $\times$ CGATIt Cleaves the Phosphodiester Backbone Between the T and the Cleaves