A DNA fragment produced by the restriction enzyme SalI is isolated from a digestion of mouse DNA.You cloned the fragment by first ligating it into a unique Sal I site found in a 3.5 kb cosmid.The ligation into the SalI site regenerates a SalI cut site at each end of the cloned fragment.You decide to determine the restriction map of this fragment.You digest the fragment with different restriction enzymes (below).
Note: The cosmid used in this strategy does not have EcoRI or HindIII sites (but the cloned fragment apparently does).
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