Suppose you cut two different DNAs,

$$\begin{array} { l l }
\text { one wih BamH1: } & \text { 5'-GGATCC-3' } \
& \text { 3'-CCTAGG-5' } \\
\text { and one with BglII: } & \text { 5'-A GATCT-3' } \
& \text { 3'-TCTAG A-5' }
\end{array}$$

then ligate them together through their compatible sticky ends.Once joined,could you separate these two DNAs again with either restriction enzyme? Why or why not? (Each of these enzymes is cut between the first two nucleotides,at the 5? end of each strand of the palindrome.)

Question

Suppose you cut two different DNAs,

$$\begin{array} { l l } 
\text { one wih BamH1: } & \text { 5'-GGATCC-3' } \
& \text { 3'-CCTAGG-5' } \\
\text { and one with BglII: } & \text { 5'-A GATCT-3' } \
& \text { 3'-TCTAG A-5' }
\end{array}$$

then ligate them together through their compatible sticky ends.Once joined,could you separate these two DNAs again with either restriction enzyme? Why or why not? (Each of these enzymes is cut between the first two nucleotides,at the 5? end of each strand of the palindrome.)

Quizplus · Accepted Answer

The Answer of Suppose you cut two different DNAs,

$$\begin{array} { l l } 
\text { one wih BamH1: } & \text { 5'-GGATCC-3' } \
& \text { 3'-CCTAGG-5' } \\
\text { and one with BglII: } & \text { 5'-A GATCT-3' } \
& \text { 3'-TCTAG A-5' }
\end{array}$$

then ligate them together through their compatible sticky ends.Once joined,could you separate these two DNAs again with either restriction enzyme? Why or why not? (Each of these enzymes is cut between the first two nucleotides,at the 5? end of each strand of the palindrome.)

Suppose You Cut Two Different DNAs Then Ligate Them Together Through Their Compatible Sticky Ends