Although a protein's primary sequence can be inferred from the nucleotide sequence,modifications such as ______ can be determined most easily by tandem mass spectrometry followed by protein database searching.
A)phosphorylation
B)disulfide crosslinks
C)glycosylation
D)acetylation
E)all of the above

Question

Quizplus · Accepted Answer

Tandem mass spectrometry is a powerful method for identifying post-translational modifications (PTMs) that cannot be inferred from DNA or RNA sequences. PTMs like phosphorylation, disulfide crosslinks, glycosylation, and acetylation can be identified through tandem mass spectrometry followed by protein database searching. Therefore, the best choice would be E, all of the above.

Although a Protein's Primary Sequence Can Be Inferred from the Nucleotide