Protein sequences are customarily 'reconstructed' from sequenced fragments because
A)protein purification invariably results in the fragmentation of the protein of interest.
B)proteins are naturally and inevitably cleaved by proteolytic enzymes.
C)proteins are composed of multiple subunits.
D)large polypeptides cannot be directly sequenced.
E)all of the above

Question

Quizplus · Accepted Answer

Large polypeptides cannot be directly sequenced due to technical limitations, such as difficulties in obtaining a pure sample of the protein and limitations in instrumentation. Therefore, protein sequencing typically involves breaking down the protein into smaller fragments that can be sequenced and then reconstructing the original protein sequence from those fragments. While A and B may be true for some proteins, they do not apply to all proteins, and C is not necessarily a reason for protein reconstruction through sequencing.

Protein Sequences Are Customarily 'Reconstructed' from Sequenced Fragments Because