Conventional one dimensional NMR spectroscopy is not generally an effective tool for determination of protein structure because…
(I)Proteins (including small proteins)have a high number of hydrogen atoms.
(II)NMR requires a high quality protein crystal.
(III)The NMR spectra exhibit high peak overlap.
A)I and II
B)II and III
C)I and III
D)I,II,and III
E)III only

Question

Quizplus · Accepted Answer

One dimensional NMR spectroscopy is not generally an effective tool for determination of protein structure because (I) proteins have a high number of hydrogen atoms, making spectra complex and difficult to interpret, and (III) the NMR spectra exhibit high peak overlap, making it difficult to assign specific peaks to particular nuclei. High quality protein crystals are not required for NMR spectroscopy of proteins in solution, and therefore statement (II) is incorrect.

Conventional One Dimensional NMR Spectroscopy Is Not Generally an Effective