Enzyme E is responsible for conversion of substrate X to product U.As a result of this conversion electrons are transported to a coenzyme (FAD) within Enzyme E.In order for the reaction to be completed,a second substrate NAD+ must also bind Enzyme E and collect stored electrons (which converts it to product,NADH) .The graph below shows the data while varying X,with fixed concentrations of NAD+.What type of multi-substrate mechanism does enzyme E utilize?
Substrates: X NAD+
Products: U NADH
A) sequential - Ordered
B) sequential - Random
C) simultaneous addition
D) Ping Pong
E) Sequential but the data cannot differentiate between ordered and random.
Correct Answer:
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