Which of the following is used to allow effective gel electrophoresis of DNAs 100,000 base pairs?
A)hydroxyapatite treatment prior to gel loading
B)Northern blots
C)pulsed-field gel electrophoresis
D)Southern Blot
E)Western Blot

Question

Which of the following is used to allow effective gel electrophoresis of DNAs > 100,000 base pairs?
A)hydroxyapatite treatment prior to gel loading
B)Northern blots
C)pulsed-field gel electrophoresis
D)Southern Blot
E)Western Blot

Quizplus · Accepted Answer

Pulsed-field gel electrophoresis (PFGE) is a type of gel electrophoresis that allows effective separation and analysis of large fragments of DNA that are > 100,000 base pairs. This is achieved through the use of alternating electric fields that continuously change the direction of the current, resulting in a longer migration distance for the DNA fragments, allowing for better resolution. Therefore, PFGE is the best choice for this question.

Which of the Following Is Used to Allow Effective Gel