The advantage of using an SDS-PAGE gel compared with a native PAGE gel is that the SDS-PAGE gel
A) separates proteins only based on molar mass.
B) gives information about the charge or conformation of the protein.
C) results in a better separation of small proteins.
D) increases the resolution of large and small proteins.

Question

Quizplus · Accepted Answer

SDS-PAGE gel separates proteins solely based on their molar mass by denaturing and uniformly negatively charging all proteins through the addition of SDS. Native PAGE, however, separates proteins based on both molar mass and charge or conformation. Therefore, SDS-PAGE results in a better separation of proteins of different sizes, including both large and small proteins.

The Advantage of Using an SDS-PAGE Gel Compared with a Native